DNA of bacteria in beagle feces - SRA

SRX26747536: DNA of bacteria in beagle feces
1 ILLUMINA (Illumina NovaSeq 6000) run: 4.3M spots, 1.3G bases, 329.6Mb downloads

Design: DNA of LSbg3 strain was extracted and DNA concentration, purity and integrity were tested by Agilent 5400. Genome sequencing of LSbg3 was conducted by Novo on Illumina platform PE150 to remove low-quality bases and obtain Clean Data using SOAP denovo(version 2.04),SPAdes,ABySS assembly software for assembly, and CISA software for integration using gapclose(Version: 1.12) and other software optimized the preliminary assembly results and filled holes, so as to obtain the final assembly results. GeneMarkS(Version 4.17) were used to predict the coding genes of the sequenced genomes; Rfam(Version 1.2) and tRNAscan-SE(Version 1.3.1) were used to predict rRNA trnas and other Ncrnas, respectively. Functional annotations were obtained from the KEGG GO COG and SwissProt databases Prediction enzymes from the CAZy(Carbohydrate Active Enzymes,cazymes) database identified virulence factors and antibiotic resistance genes based on the core dataset of pathogen virulence factors (VFDB) and the Integrated Antibiotic Resistance Database (CARD)

Submitted by: Sichuan Agricultural University

Study: Study on the mechanism of action of canine lactic acid bacteria LSbg3 with good stress resistance on functional diarrhea in dogs

PRJNA1187351 • SRP545785 • All experiments • All runs

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Therefore, this study aims to address this gap by characterizing lactic acid bacteria strains isolated from dog feces at the genotypic level, focusing on their potential as stress-resistant probiotics. Our research can make a significant contribution to the development of effective and safe probiotics.

Sample:

SAMN44790508 • SRS23236269 • All experiments • All runs

Organism: Lactobacillus

Library:

Name: LSbg3-1

Instrument: Illumina NovaSeq 6000

Strategy: WGS

Source: GENOMIC

Selection: PCR

Layout: PAIRED

Runs: 1 run, 4.3M spots, 1.3G bases, 329.6Mb

Run	# of Spots	# of Bases	Size	Published
SRR31373945	4,315,809	1.3G	329.6Mb	2024-11-18

ID:: 36150609

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